EC Number   |
Protein Variants |
Reference |
|---|
   3.6.1.17 | P133F |
3fold decrease in kcat value |
733450 |
| 3.6.1.17 | R54Q |
fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys. Cleavage of (2'-pdA)AppppA to produce ATP and 2'-deoxyadenylated AMP rather than 2'-deoxyadenylated ATP + AMP as with wild-type enzyme, the ratio of ATP released is increased compared to wild-type. 330fold reduction in sensitivity to fluoride |
654489 |
| 3.6.1.17 | T70A |
active site mutant, has no effect on platelet aggregation and secretion |
733563 |
| 3.6.1.17 | Y121A |
turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 8.5fold higher than the KM-value of the wild-type enzyme |
656173 |
| 3.6.1.17 | Y27A |
turnover-number for hydrolysis of Ap4A is 113% of that of the wild-type enzyme, KM-value is 1.25fold higher than the KM-value of the wild-type enzyme |
656173 |
| 3.6.1.17 | Y27D |
turnover-number for hydrolysis of Ap4A is 126% of that of the wild-type enzyme, KM-value is 1.8fold higher than the KM-value of the wild-type enzyme |
656173 |
| 3.6.1.17 | Y76A |
turnover-number for hydrolysis of Ap4A is 8.7% of that of the wild-type enzyme, KM-value is 6.7fold higher than the KM-value of the wild-type enzyme |
656173 |
| 3.6.1.17 | Y76A/Y121A |
turnover-number for hydrolysis of Ap4A is 4.8% of that of the wild-type enzyme, KM-value is 3.75fold higher than the KM-value of the wild-type enzyme |
656173 |
