EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.B30 | D126C |
reduced ability to form a homodimer and a heterodimer with UmuD' |
651622 |
| 3.4.21.B30 | D20Y |
slight increase in activation rate by cleavage |
652782 |
| 3.4.21.B30 | D32C |
deficiencies in RecA-mediated cleavage as well as in UV mutagenesis, less than 30% of the wild-type activity |
651629 |
| 3.4.21.B30 | D3A |
the UmuD variant is non-cleavable but is a partial biological mimic of the cleaved form UmuD |
709543 |
| 3.4.21.B30 | D91A |
the mutant is soluble and purifies as the wild type UmuD |
-, 681885 |
| 3.4.21.B30 | D91K |
site-directed mutagenesis, the mutation abolishes the interaction between the enzyme and the DNA polymerase III alpha subunit |
732588 |
| 3.4.21.B30 | E11V/I12V/V13K |
supports ClpXP degradation of UmuD' |
653588 |
| 3.4.21.B30 | E35C |
deficiencies in RecA-mediated cleavage as well as in UV mutagenesis, less than 30% of the wild-type activity |
651629 |
| 3.4.21.B30 | F15A |
slight decrease of induced mutagenesis compared to wild-type |
669529 |
| 3.4.21.B30 | F15L |
no change in activation rate by cleavage |
652782 |
