EC Number   |
Protein Variants |
Reference |
|---|
    2.7.7.62 | H46N |
site-directed mutagenesis |
644438 |
| 2.7.7.62 | I144D/F146D/Y163R |
monomer/dimer/aggregate ratio: 77/23/0. Specific activity (pmol/min/microgram): 156 (monomer), 49 (dimer) |
724319 |
| 2.7.7.62 | K19A |
in vitro activity relative to wild-type: 16%, monomer/dimer ratio: 36/64 |
-, 724319 |
| 2.7.7.62 | L38D/K41D |
monomer/dimer/aggregate ratio: 53/25/22. Specific activity (pmol/min/microgram): 125 (monomer), 120 (dimer), 78 (aggregate) |
724319 |
| 2.7.7.62 | L38K/K41D |
monomer/dimer/aggregate ratio: 52/26/22. Specific activity (pmol/min/microgram): 124 (monomer), 119 (dimer), 85 (aggregate) |
724319 |
| 2.7.7.62 | N177R |
in vitro activity relative to wild-type: 5%, monomer/dimer ratio: not determined |
724319 |
| 2.7.7.62 | N179D |
in vitro activity relative to wild-type: 95%, monomer/dimer ratio: not determined |
724319 |
| 2.7.7.62 | P20A |
in vitro activity relative to wild-type: 93%, monomer/dimer ratio: 63/37 |
724319 |
| 2.7.7.62 | P54A |
in vitro activity relative to wild-type: 62%, monomer/dimer ratio: 50/50 |
724319 |
| 2.7.7.62 | P94A |
in vitro activity relative to wild-type: 43%, monomer/dimer ratio: 52/48 |
724319 |
