EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.39 | Q66R |
increase in the malonyl transferase activities |
705648 |
| 2.3.1.39 | Q67A |
complete loss of the malonyl transferase activities |
705648 |
| 2.3.1.39 | Q67G |
complete loss of the malonyl transferase activities |
705648 |
| 2.3.1.39 | Q67R |
increase in the malonyl transferase activities |
705648 |
| 2.3.1.39 | Q9A |
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme |
661061 |
| 2.3.1.39 | R113A |
site-directed mutagenesis, inactive mutant |
735606 |
| 2.3.1.39 | R115A |
mutant shows a reduction in activity |
719494 |
| 2.3.1.39 | R117A |
the variant synthesizes acetyl-[acyl carrier protein], succinyl-[acyl carrier protein], isobutyryl-[acyl carrier protein], 2-butenoyl-[acyl carrier protein], and beta-hydroxybutyryl-[acyl carrier protein] among others from holo-acyl carrier protein and the corresponding acyl-CoAs |
755871 |
| 2.3.1.39 | R117A |
unlike the wild-type enzyme that is highly specific for malonyl-CoA to produce malonyl-[acyl-carrier protein] (ACP), the R117A variant synthesizes acetyl-ACP, succinyl-ACP, isobutyryl-ACP, 2-butenoyl-ACP, and beta-hydroxybutyryl-ACP among others from holo-ACP and the corresponding acyl-CoAs with specific activities from 3.7 to 120 nmol/min*mg |
755871 |
| 2.3.1.39 | R122A |
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme |
661061 |
