EC Number   |
Protein Variants |
Reference |
|---|
  1.7.1.17 | W105F |
31fold decrease in vmax value, Km value similar to wild-type |
-, 745718 |
| 1.7.1.17 | W105G |
complete loss of both affinity for FMN and enzyme activity |
745718 |
| 1.7.1.17 | W105H |
8fold decrease in vmax value, Km value similar to wild-type |
-, 745718 |
| 1.7.1.17 | W105Q |
68fold decrease in vmax value, Km value similar to wild-type |
-, 745718 |
| 1.7.1.17 | W105Y |
22% reduction in vmax value |
-, 745718 |
| 1.7.1.17 | W60D |
the mutant retains more than 10% of wild type activity |
-, 763801 |
| 1.7.1.17 | Y119A |
the mutant shows 59% activity with Methyl Red and 44% activity with NADH compared to the wild type enzyme |
765245 |
| 1.7.1.17 | Y120A |
site-directed mutagenesis |
742834 |
| 1.7.1.17 | Y127F |
site-directed mutagenesis, Diffraction-quality crystals for the Y127F mutant cannot be obtained |
-, 763965 |
| 1.7.1.17 | Y151F |
site-directed mutagenesis, structure comparison with the wild-type enzyme |
-, 763965 |
