EC Number |
Protein Variants |
Reference |
---|
1.1.1.B52 | K202N/L224M |
89.7% enantiomeric excess for (R)-3-quinuclidinol production, 90% conversion |
738052 |
1.1.1.B52 | K202Q/L224W |
95.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion |
738052 |
1.1.1.B52 | K202R/L224W |
94.7% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion |
738052 |
1.1.1.B52 | K202S/L224Y |
89.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion |
738052 |
1.1.1.B52 | N162A |
93.8% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion |
738052 |
1.1.1.B52 | R196A |
activity is 76% compared to activity of wild-type enzyme |
729146 |
1.1.1.B52 | R196A |
site-directed mutagenesis, the mutant shows 34% reduced activity compared to the wild-type enzyme |
741816 |
1.1.1.B52 | Y216V |
activity is 31% compared to activity of wild-type enzyme |
729146 |
1.1.1.B52 | Y216V |
site-directed mutagenesis, the mutant shows 69% reduced activity compared to the wild-type enzyme |
741816 |