EC Number   |
Protein Variants |
Reference |
|---|
    4.3.1.2 | Q73A |
analysis carried out with mutant Q73A. The potential of the mutant Q73A enzyme is shown for application in the asymmetric synthesis of a large variety of valuable N-substituted l-aspartic acids |
729599 |
| 4.3.1.2 | Q73A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 37% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 91:9 (89:11 wild-type) |
729745 |
| 4.3.1.2 | Q73A |
mutant displays significant decrease in ammonia addition and a large increase in methylamine addition compared to wild-type. Mutant displays a wide nucleophile scope including structurally diverse linear and cyclic allkylamines |
730442 |
| 4.3.1.2 | Q73N |
mutant shows very low-level amination activity preventing the measurement of kinetic parameters. Conversion 2h: 18% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | T360A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 38% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 93:7 (89:11 wild-type) |
729745 |
| 4.3.1.2 | T360S |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 76% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 86:14 (89:11 wild-type) |
729745 |
| 4.3.1.2 | Y356A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 24% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
