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<< < Results 21 - 26 of 26
EC Number
Amino acid exchange
Commentary
Reference
more
construction of an isogenic inactive mutant strain M6
T379A
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type
T379V
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type
V189A
protease activity (microgram/min/mg) substrate azocasein: 2.6, substrate proSPE B C47S mutant: 0.9, compared to wild-type 341 and 378, respectively
W212A
protease activity (microgram/min/mg) substrate azocasein: 1.2, substrate proSPE B C47S mutant: 0.9, compared to wild-type 341 and 378, respectively
W214A
protease activity (microgram/min/mg) substrate azocasein: 0.8, substrate proSPE B C47S mutant: 0.8, compared to wild-type 341 and 378, respectively
<< < Results 21 - 26 of 26