metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone
pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type
semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico.
the mutant shows no butyrate kinase II activity
the mutant shows 21% of wild type butyrate kinase II activity
the mutant shows 0.38% of wild type butyrate kinase II activity
the mutant shows 0.44% of wild type butyrate kinase II activity