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<< < Results 11 - 17 of 17
EC Number
Amino acid exchange
Commentary
Reference
more
metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone
more
pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type
more
semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico.
R150F
the mutant shows no butyrate kinase II activity
R150K
the mutant shows 21% of wild type butyrate kinase II activity
R215F E332D
the mutant shows 0.38% of wild type butyrate kinase II activity
R215K
the mutant shows 0.44% of wild type butyrate kinase II activity
<< < Results 11 - 17 of 17