7.6.2.5 | purified recombinant His-tagged purified CgHmuT from enzyme complex HmuTUV in the holo-form, hanging drop vapour diffusion method, mixing of 50 mg/ml protein in 50 mM Tris-HCl, pH 8.5, and 200 mM NaCl with precipitant solution containing 2.1 M ammonium sulfate and 0.2 M potassium thiocyanate, X-ray diffraction structure determination and analysis at 1.42 A resolution |
750302 |