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Search Crystallization (Commentary)

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EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 6.2.1.16purified recombinant enzyme SlAacS complexed with AMP and acetoacetate, hanging drop vapor diffusion method, mixing of 11 mg/ml protein and 1.5fold molar excess of buffered AMP and acetoacetate with a reservoir solution containing 0.7-1.2M K3citrate and 50 mM BTP-HCl, pH 7.0, 14°C, 1 week, X-ray diffraction structure determination and analysis, molecular replacement using full-length Salmonella enterica acetyl-CoA synthetase, PDB ID1PG4, stripped of waters and cofactors/substrates as search model 746014
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