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EC Number	Crystallization (Commentary)
6.2.1.12	isoform 4CL2 in complex with Mg2+ and ATP or with AMP and coenzyme A or with three different hydroxycinnamate-AMP intermediates: 4-coumaroyl-AMP, caffeoyl-AMP, and feruloyl-AMP, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M potassium nitrate or 4.4% (w/v) PEG 8000, 0.08 M sodium cacodylate (pH 6.5), 0.16 M calcium acetate, and 20% (v/v) glycerol or 25.5% (w/v) PEG 8000, 0.085 M sodium cacodylate (pH 6.5), 0.17 M sodium acetate, and 15% (v/v) glycerol or 25% (w/v) PEG 3350, 0.2 M ammonium acetate, and 0.1 M Tris (pH 8.5)
6.2.1.12	purified recombinant isozyme At4CL1 and engineered isozyme At4CL1-VvSTS fusion enzyme, X-ray diffraction structure determination and analysis

EC Number

Crystallization (Commentary)

6.2.1.12

isoform 4CL2 in complex with Mg2+ and ATP or with AMP and coenzyme A or with three different hydroxycinnamate-AMP intermediates: 4-coumaroyl-AMP, caffeoyl-AMP, and feruloyl-AMP, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M potassium nitrate or 4.4% (w/v) PEG 8000, 0.08 M sodium cacodylate (pH 6.5), 0.16 M calcium acetate, and 20% (v/v) glycerol or 25.5% (w/v) PEG 8000, 0.085 M sodium cacodylate (pH 6.5), 0.17 M sodium acetate, and 15% (v/v) glycerol or 25% (w/v) PEG 3350, 0.2 M ammonium acetate, and 0.1 M Tris (pH 8.5)

6.2.1.12

purified recombinant isozyme At4CL1 and engineered isozyme At4CL1-VvSTS fusion enzyme, X-ray diffraction structure determination and analysis

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Release 2023.1 (January 2023)