EC Number   |
Reference |
|---|
    6.1.1.27 | hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.0, 0.3M ammonium sulfate, 36% (w/v) 2-methyl-2,4-pentanediol |
745864 |
| 6.1.1.27 | hanging-drop vapor diffusion, 3.2 A resolution |
694852 |
| 6.1.1.27 | SepRS tetramer in complex with tRNACys and O-phosphoserine, selenomethionine SAD method, and SepRS-tRNACys binary complex, 0.001 ml of 6-8 mg/ml protein in 10 mM Tris-HCl buffer, pH 8.0, 5 mM MgCl2, 150 mM NaCl and 5 mM 2-mercaptoethanol, and 2 mM O-phospho-L-serine, mixed with 0.001 ml reservoir solution containing 8% w/v PEG 6000 and 1.2 M NaCl, 20°C, cryoprotection by 22% v/v glycerol, X-ray diffraction structure determination and analysis at 2.6 A and 2.8 A resolution, respectively, modeling, determination of crystal structures of SepRS(E418N/E420N)-tRNAOpal-O-phosphoserine and SepRS(E418N/E420N)-tRNAAmber-O-phosphoserine at 3.2 and 3.3 resolutions, respectively |
676139 |
| 6.1.1.27 | sitting drop vapor diffusion method, using 0.1 M MES pH 5.3, 2.37 M ammonium sulfate |
743961 |
| 6.1.1.27 | tRNA-free SepRS, hanging drop vapor diffusion method, 0.001 ml of protein solution mixed with 0.001 ml reservoir solution containing 11.25% w/v PEG 4,000, 75 mM sodium citrate, 75 mM N-(2-acetamido)iminodiacetic acid-NaOH buffer, pH 6.7, versus 1 ml reservoir solution, 20°C, cryoprotection by 22% v/v glycerol, X-ray diffraction structure determination and analysis at 3.6 A resolution, modeling |
676139 |
