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EC Number Crystallization (Commentary)
Show all pathways known for 6.1.1.16Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.16hanging drop vapor diffusion method, crystals of enzyme bound to tRNACys at a resolution of 2.3 A
Show all pathways known for 6.1.1.16Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.16purified enzyme complexed with ATP and cysteine, hanging-drop vapour diffusion from ammonium sulfate precipitant containing solution, X-ray diffraction structure analysis at 2.7 A resolution
Show all pathways known for 6.1.1.16Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.16purified recombinant free enzyme or cysteine and ATP complexed enzyme, hanging-drop vapour diffusion method, 17°C, 0.001 ml protein solution: 6 mg/ml enzyme, 10 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 mM ATP, 10 mM cysteine, plus equal volume of reservoir solution: 0.1 M sodium cacodylate, pH 6.5, 15-17% PEG 8000, 0.2 M magnesium acetate, 2% tert-butanol, 1-3 weeks, cysteine but not ATP is required for crystal growth, X-ray diffraction structure determination at 2.3-3.0 A resolution, and analysis
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