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Results 1 - 7 of 7
EC Number Crystallization (Commentary) Reference
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18hanging-drop method, crystals grow best at 295 K with the optimized mother-liquor conditions of 21-23% PEG 4K, 0.19 M ammonium acetate and 90 mM citrate, pH 5.25-5.5. Crystals belong to space group I422, with unit-cell parameters a = 99.25, c = 164.81 A 660590
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18hanging-drop vapor-diffusion method, crystals grown in the presence of 4-carboxaminoimidazole ribonucleotide belong to space group P2(1)2(1)2(1), with unit cell parameters a = 86.92, b = 94.55 and c = 149.96 A. The 1.5 A crystal structure reveals an octameric structure with 422 symmetry 663416
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18multiple REDOR NMR studies of a 151000 Da complex of uniformly 15N-labeled enzyme and the active site ligand [6-13C]-citrate show a single ionization equilibrium associated with the key histidine H59. H59 exists in approximately equimolar amounts of an Ndelta-unprotonated pyridine-like form and an Ndelta-protonated pyrrole-like form. Proton transfer mechanism involves H59 Ndelta 678322
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18mutant H59N soaked in 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, protonation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate C4 may occur in absence of H59 678203
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18nanodroplet vapor diffusion method, 1.77 A resolution, unit cell parameters: a = b = 103.25 A, c = 65.45 A, alpha = beta = 90° 663353
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18purified recombinant detagged enzyme, crystallization at room temperature, 10 mg/ml protein is mixed with a reservoir solution consisting of 0.1 M sodium acetate trihydrate, pH 4.5, and 2 M ammonium sulfate, 24 h, X-ray diffraction structure determination and analysis at 1.45 Aresolution, molecular replacement using the structure of Bacillus anthracis PurE, PDB ID 1xmp, as template 726578
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18wild-type cocrystallized with 4-nitroaminoimidazole ribonucleotide and mutants H45N and H45Q soaked with 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate 678260
Results 1 - 7 of 7