EC Number   |
Reference |
|---|
    5.4.99.16 | crystals are grown by vapor diffusion. Structure of the Mycobacterium smegmatis TreS:Pep2 complex, containing trehalose synthase (TreS) and maltokinase (Pep2), which converts trehalose to maltose 1-phosphate as part of the TreS:Pep2-GlgE pathway. The structure, at 3.6 A resolution, reveals that a diamond-shaped TreS tetramer forms the core of the complex and that pairs of Pep2 monomers bind to opposite apices of the tetramer in a 4 + 4 configuration |
759499 |
| 5.4.99.16 | enzyme complexed with alpha-acarbose, Ca2+, Cl-, and Mg2+ or with Cl-, Ca2+, and Mg2+, PDB IDs 3ZOA and 3ZO9, X-ray diffraction structure determination and analysis at 1.85 and 1.84 A resolution, respectively |
746863 |
| 5.4.99.16 | enzyme complexed with Ca2+, glycerin, and sulfate ion, PDB ID 4LXF, X-ray diffraction structure determination and analysis at 2.6 A resolution |
746863 |
| 5.4.99.16 | enzyme complexed with Ca2+, Mg2+, and tromethamine, PDB ID 4TVU, X-ray diffraction structure determination and analysis at 2.7 A resolution |
746863 |
| 5.4.99.16 | enzyme, PDB ID 5X7U, X-ray diffraction structure determination and analysis at 2.5 A resolution |
746863 |
| 5.4.99.16 | free enzyme and enzyme in complex with inhibitor acarbose, hanging drop vapor diffusion technique, mixing of 0.002 ml of 20 mg/ml protein in 40 mM sodium phosphate buffer, pH 6.0, with 0.002 ml of reservoir solution containing 0.1 M sodium cacodylate, pH 6.5, 0.2 M MgCl2, and 10-14% PEG 1000, 20°C, 1 day to 1 week, X-ray diffraction structure determination and analysis at 1.84 A resolution, molecular replacement |
727598 |
| 5.4.99.16 | purified recombinant enzyme mutant N253F, hanging drop vapour diffusion method, mixing of 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, 100 mM NaCl, 3.3% glycerol, and 1 mM DTT, with 0.0.02 ml of reservoir solution containing 0.3 M Tris-HCl pH 7.0, 7% PEG 4000, and 0.2 M sodium acetate trihydrate, and equilibration against 0.5 ml of reservoir solution, 15°C, 2-3 weeks, X-ray diffraction structure determination and analysis |
746668 |
| 5.4.99.16 | purified recombinant wild-type and N253A mutant enzymes in complex with inhibitor Tris, hanging drop vapour diffusion method, 15°C, for the wild-type enzyme, mixing of 0.002 ml of 30 mg/ml protein in 20 mM sodium phosphate, pH 7.4, with 0.002 ml of reservoir solution containing 9% PEG 4000, 0.2 M sodium acetate trihydrate, 0.3 M Tris-HCl, pH 8.5, 6-8 weeks, for the mutant enzyme, mixing of 0.002 ml of 60 mg/ml protein in 20 mM sodium phosphate, pH 7.4, with 0.002 ml of reservoir solution containing 11% PEG 4000, 0.2 M sodium acetate trihydrate, 0.3 M Tris-HCl, pH 8.5, and 5% glycerol, 2 weeks, X-ray diffracion structure determination and analysis at 2.21-2.70 A resolution |
746621 |
