EC Number |
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5.4.99.11 | crystal structure determination, PDB ID 1M53 |
5.4.99.11 | crystal structure determination, PDB IDs GBD and GBE, with ethylene glycol, citrate, 1,2-ethanediol, and 1-deoxynojirimycin as interacting compounds |
5.4.99.11 | crystal structure determinations of enzyme mutants F164L and R284C, PDB IDs 4H2C, 4GIN, 4GI6, 4GI8, 4GIA, and 4GI9, interacting compounds are Ca2+, glycerin, D-glucose, and Tris |
5.4.99.11 | crystal structure determinations of native enzyme and enzyme mutants D200A and E254Q, PDB IDs 1ZJB, 2PWH, 1ZJA, 2PWD, 2PWG, 2PWF, and 2PWE, interacting compounds are Ca2+, sucrose, D-glucose, tromethamine, Tris, 1-deoxynojirimycin, and castanospermine |
5.4.99.11 | expression in Escherichia coli |
5.4.99.11 | hanging-drop vapor diffusion method, structure solved at 2.2 A resolution |
5.4.99.11 | hanging-drop vapour-diffusion method at 295 K |
5.4.99.11 | in complex with deoxynojirimycin, sitting drop vapor diffusion method, using |
5.4.99.11 | native enzyme, crystals belong to space group P212121 and diffract to 1.95 A resolution |
5.4.99.11 | purified recombinant enzyme mutants R284C in complex with transition-state mimic deoxynojirimycin, and F164L in complex with glucose, sucrose, trehalulose, or isomaltulose, X-ray diffraction structure determination and analysis at 1.7-2.15 A resolution |