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Crystallization (Commentary)
5.3.1.13
mutant enzyme K59A, sitting drop vapor diffusion method, using 20% PEG 8K and 0.1 M HEPES, pH 7.5
5.3.1.13
to 1.7 A resolution, a tetramer of a single-domain sugar isomerase with an endogenous ligand, cytidine 5'-monophosphate-3-deoxy-D-mannooct-2-ulosonate, bound at the active site. Cytidine 5'-monophosphate-3-deoxy-D-mannooct-2-ulosonate corresponds to the end product in the 3-deoxy-D-mannooct-2-ulosonate biosynthetic pathway and presumably acts as a feedback inhibitor
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