5.1.3.9 | purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.0015 ml of crystallization solution containing 1 M trisodium citrate, 0.1 M sodium cacodylate, pH 6.5, 1 day, 8°C, X-ray diffraction structure determination and analysis at 1.84 A resolution, molecular replacement using monomeric enzyme structure, PDB ID 1y0e, as search template |
746647 |