    5.1.3.13 | purified mixture containing all four dTDP-L-rhamnose biosynthetic enzymes, RfbA, RfbB, RfbC and RfbD, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution, containing 10mM Tris-HCl, pH 8.3, 500 mM NaCl, 5 mM 2-mercaptoethanol, 1 mM dTTP, 1 mM Glc1P, 1 mM NAD+, 1 mM NADP+, and 1mM MgCl2, with 0.001 ml of reservoir solution containing 0.2 M diammonium citrate, pH 5.0, 20% w/v, and PEG 3350, at room temperature, X-ray diffraction structure determination and analysis at 1.63 A resolution, molecular replacement using one monomer from PDB ID 1dzr as the search model |
746670 |
