EC Number   |
|---|
    4.2.1.75 | 5 mg/ml purified recombinant wild-type and selenomethionine-enzyme, sitting drop vapour diffusion method, 4°C, equal volumes of protein solution, containing 10 mM Tris-HCl, pH 7.5, 1 mM dithiothreitol, and reservoir solution, containing 20% 2-methyl-2,4-pentanediol, 100 mM MES, pH 6.0, X-ray diffraction structure determination and analysis at 1.85-2.1 A resolution |
| 4.2.1.75 | purified recombinant enzyme, three apoenzyme forms, U3S1, U3S2, U3S3, and one enzyme-product complex form, vapor diffusion in sitting drop method, mixing 0.002 ml of protein with 0.002 ml of precipitant solution and equilibration over 1 mL of precipitant solution of different compositions, for U3S1, 1.2 M NaH2PO4, 0.8 M K2H2PO4, for U3S2, 21-24% PEG 8000, 0.2 M Mg-acetate, 0.1 M MES-HCl, pH 6.5, 0.2 M NaCl, and for U3S3, 18-24% PEG 8000, 0.1 M Tris-HCl, pH 8.5, 0.2 mM MgCl2, 4°C, 1-7 days, X-ray diffraction structure determination and analysis at 1.6-2.0 A resolution |
| 4.2.1.75 | purified wild-type recombinant His6-tagged U3S and selenomethionine-substituted derivative, 0.001 ml of 20 mg/ml protein in 20 mM Hepes, pH 7.2, 20 mM NaCl, is mixed with 0.001 ml of reservoir solution containing 20% PEG 3350, 0.2 M tri-Na citrate, and equilibrated against 0.1 ml of reservoir solution, about a month at 8°C and followed by microseeding method, X-ray diffraction structure determination and analysis at 2.5 A resolution, single wavelength anomalous dispersion method |
