   4.2.1.126 | purified recombinant enzyme as apoenzyme and in complex with inhibitor (2R)-2-[[(2R,3R,4R)-5-(acetylamino)-2,3,7-trihydroxy-1-(phosphonooxy)heptan-4-yl]oxy]propanoic acid, sitting drop vapor diffusion method, mixing of 8 mg/mL protein in 200 mM NaCl, 50 mM HEPES, and 5% glycerol with 0.2 M NaCl, 0.1 M Bis-Tris-HCl, pH 5.5, and 25% w/v PEG 3350 or with 0.17 M ammonium sulfate, 25.5% w/v PEG 4000, and 15% v/v glycerol, the former gives better results, soaking of crystals in solution containing the inhibitor, 25°C, X-ray diffraction structure determinnation and analysis at 2.6 A and 2.4 A resolution, respectively, molecular replacement using PDB entry 1NRI as the template |
729247 |
