EC Number   |
|---|
   4.1.2.61 | 1.8 A resolution, data collection and refinement statistics shown, structure solution and in silico modelling determined, monomer folding and factors affecting trimer assembly indicated, oligo structure and modelling of the active site determined |
| 4.1.2.61 | by the hanging-drop method of vapour diffusion using polyethylene glycol 20000 Da as the precipitant, 1.8 A resolution |
| 4.1.2.61 | hanging-drop vapour diffusion method, wild-type hydroxycinnamoyl-CoA hydratase/lyase in complex with acetyl-CoA and vanillin 2vss: protein concentration of 10 mg/ml in 11% (w/v) PEG [poly(ethylene glycol)] (20000 Da) with 8% PEG (550 Da) monomethyl ether, 0.8 M sodium formate and 0.2% (v/v) butane 1,4-diol in 0.05 M Mes buffer (pH 5.6), 10 mM feruloyl.CoA, resolution 2.22 A, space group P 21 21 21. A ternary complex of hydroxycinnamoyl-CoA hydratase/lyase mutant S123A (HCHL) with acetyl-CoA and vanillin gives insights into substrate specificity and mechanism 2vsu: resolution 1.9 A, space group P 21 21 21 |
| 4.1.2.61 | sitting drop vapor diffusion method, crystal structure is solved and refined at 2.1 A resolution |
