EC Number |
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4.1.1.32 | enzyme alone and in complexes with the non-hydrolyzable GTP analog beta,gamma-methylene GTP, and with phosphoenolpyruvate, hanging-drop vapor-diffusion method, X-ray analysis |
4.1.1.32 | hanging drop vapour diffusion method using 0.1 M HEPES (pH 7.4) and 19% PEG 6000 |
4.1.1.32 | hanging drop vapour diffusion method, at 25 ?C in 0.1 M HEPES (pH 7.4) and 12-30% PEG 3350, creating PEPCK-Mn2+, PEPCK-Mn2+-oxaloacetate, PEPCK-Mn2+-oxaloacetate-Mn2+GDP, and PEPCKMn2+-Mn2+GTP crystals |
4.1.1.32 | hanging drop vapour diffusion method. Crystallizes in space group P21 with four molecules per asymmetric unit. The 2.3 A resolution structure is solved by molecular replacement using the human cytosolic PCK |
4.1.1.32 | mutant enzyme A467G in complex with Mn2+, substrates and inhibitors |
4.1.1.32 | X-ray analysis, hanging-drop method |