EC Number   |
|---|
    4.1.1.20 | co-crystals of DAPDC and azelaic acid and L-lysine at 2.6 A and 2.0 A resolution, respectively, hanging drop vapor-diffusion, 12% polyethylene glycol 6000, 100 mm Tris-Cl, pH 8.0, 20 mM MgCl2 and 10 mM L-lysine |
| 4.1.1.20 | hanging drop vapor diffusion method, using 20-23% (w/v) PEG-MME 5000, 0.1 M MES pH 6.1-6.6, and 60 mM (NH4)2SO4 |
| 4.1.1.20 | hanging drop vapour diffusion method, in 100 mM bicine, pH 8.0, and 1.6 M ammonium sulfate, at 4°C |
| 4.1.1.20 | hanging-drop vapour-diffusion method, crystals grown in absence of substrate or cofactor are colourless and appear to contain one homotetramer of LysA in the asymmetric unit |
| 4.1.1.20 | purified recombinant enzyme complexed with pyridoxal 5'-phosphate cofactor alone or with pyridoxal 5'-phosphate cofactor and product L-lysine, sitting drop vapor diffusion method, mixing of 0.001 ml of 60 mg/ml protein in 40 mM Tris-HCl, pH 8.0, and 150 mM NaCl with 0.001 ml of reservoir solution, containing 0.8 M sodium citrate tribasic and 0.1 M sodium cacodylate, pH 6.5, and equilibration against 005 ml of reservoir solution, soaking of crystals in 10 mM ligand-containing mother liquor, X-ray diffraction structure determination and analysis at 1.72-2.40 A resolution, molecular replacement using the structure of Mycobacterium tuberculosis meso-diaminopimelate decarboxylase structure, PDB ID 1HKV, as search model, modeling |
| 4.1.1.20 | to 2.0 A resolution |
| 4.1.1.20 | to 2.3 A resolution |
| 4.1.1.20 | vapor-diffusion at room temperature, crystals are grown from 0.002 mL DDC solution in 50 mM sodium MES, pH 6.0 and 0.1 mM pyridoxal 5'-phosphate mixed with 0.002 ml well solution consisting of 1.44-1.54 M lithium sulfate, 50 mM MES, pH 6.0, and 0.1 mM pyridoxal 5'-phosphate |
| 4.1.1.20 | vapor-diffusion in hanging drops at 18°C, crystals grow within 3-7 days in 0.004 ml hanging-drops containing 0.002 ml of DAPDC at 10 mg/ml and 5 mM lysine, equilibration against 0.5 ml well solution containing 24% polyethylene glycol mono-methylester 5000, 100 mM MES, pH 6.3, and 60 mM ammonium sulfate, crystals diffract to 2.6 A |
