3.7.1.2 | inhibitor complexed enzyme, 3 mg/ml purified hanging drop vapor diffusion method, room temperature, in a 2:1 ratio with precipitant solution, containing 17-18% PEG 8000, 0.03-0.15 nickel acetate, no to 0.24 M sodium acetate, 0.1 M sodium cacodylate, pH 6.5, soaking in inhibitor 4-(hydroxymethylphosphinoyl)-3-oxo-butanoic acid solution for 16 h prior to data collection via X-ray diffraction, structure determination and analysis at 1.3 A resolution |
655997 |