EC Number |
---|
3.5.4.9 | - |
3.5.4.9 | crystal structure of DC301, the cytosolic DC domain of the trifunctional enzyme |
3.5.4.9 | crystal structure of the enzyme (MTHFD2) in complex with a substrate-based inhibitor and the enzyme cofactors NAD+ and inorganic phosphate |
3.5.4.9 | hanging drop vapor diffusion method, using 25% (w/v) PEG 3350 and 0.2 M magnesium formate |
3.5.4.9 | in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method |
3.5.4.9 | purified enzyme free and in complex with NADP+, sitting drop vapour diffusion method, mixing of 1.8 mg/ml protein in 10 mM Tris-HCl, 50 mM KCl, and 2 mM DTT, with 18% PEG 4000, 400 mM NaCl, and 100 mM Tris-HCl, pH 8.0 at 22°C, 5 mM NADP+ is added during crystallization, X-ray diffraction structure determination and analysis, molecular replacement |
3.5.4.9 | purified recombinant apo-DHCH, hanging drop vapor diffusion, mixing of 0.002 ml 9 mg/ml protein in 50 mM TrisHCl, 100 mM NaCl, pH 7.5, with 0.002 ml reservoir solution containing 20% PEG 3350 and 0.2 M ammonium acetate, equilibration against 0.07 ml of reservoir solution at 22°C, 3 days, X-ray diffraction structure determination and analysis at 2.7 A resolution, modelling |
3.5.4.9 | small platelike crystals belong to body centered orthorhombic space group I222 |