3.5.4.19 | purified untagged recombinant enzyme, hanging drop vapour diffusion method, 2 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is mixed in a 1:1 ratio with reservoir solution containing 100 mM HEPES-HCl, pH 7.5, 50 mM CdSO4, 1.6 M sodium acetate, and 10% w/v glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution |