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Results 1 - 6 of 6
EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52-
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52hanging drop vapor diffusion, crystal structure of the N-terminal domain of PNGase in complex with the cofactor-binding motif of p97 contained within the last 10 amino acid residues of the C terminus provides detailed insight into the interaction between p97 and its substrate-processing cofactors
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52purified His6-tagged wild-type and selenomethionine-labeled enzymes, hanging drop vapor diffusion method, method optimization, SeMet-labeled enzyme from 10% PEG 4000, 0.01 M MgCl2, 0.2 M KCl, and 0.05 M sodium cacodylate, pH 6.5, and native PNGase F-II crystals from 12% PEG 3350 and 0.1 M sodium malonate, pH 7.0, 16°C, X-ray diffraction structure determination and analysis at 2.8 A and 1.9 A resolution, respectively, molecular replacement using the structure of native PNGase F-II as the search model
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52purified recombinant enzyme core domain and XPCB domain in complex with the recombinant murine HR23B protein, 9.5 mg/ml of enzyme domains in a 1:1 ratio, vapour diffusion method, against reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 28-32% PEG 4000, 0.2 M sodium acetate, heavy atom derivatization by soaking in 1 mM ethyl mercury thiosalicylate, 1 mM K2[PtCN4], or 1 mM KAuCN2 for 4 h, cryoprotection by 20% glycerol, X-ray diffraction structure determination and analysis at 1.85 A resolution
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52purified wild-type PUB domain, also as selenomethionine variants, by sitting drop vapour diffusion method at 17°C, with a reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% PEG 4000, using 20% glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 1.6 A resolution, mutant L66M/L75M/L87M PUB domain are crystallized in 50% PEG 400, 0.1 M CHES, pH 9.5, 0.2 M NaCl, molecular replacement, X-ray diffraction structure determination and analysis at 1.9 A resolution
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52the crystal structure of PNGase in complex with N,N'-diacetylchitobiose is described, refined at 3.4 A
Results 1 - 6 of 6