EC Number |
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3.5.1.52 | - |
3.5.1.52 | hanging drop vapor diffusion, crystal structure of the N-terminal domain of PNGase in complex with the cofactor-binding motif of p97 contained within the last 10 amino acid residues of the C terminus provides detailed insight into the interaction between p97 and its substrate-processing cofactors |
3.5.1.52 | purified His6-tagged wild-type and selenomethionine-labeled enzymes, hanging drop vapor diffusion method, method optimization, SeMet-labeled enzyme from 10% PEG 4000, 0.01 M MgCl2, 0.2 M KCl, and 0.05 M sodium cacodylate, pH 6.5, and native PNGase F-II crystals from 12% PEG 3350 and 0.1 M sodium malonate, pH 7.0, 16°C, X-ray diffraction structure determination and analysis at 2.8 A and 1.9 A resolution, respectively, molecular replacement using the structure of native PNGase F-II as the search model |
3.5.1.52 | purified recombinant enzyme core domain and XPCB domain in complex with the recombinant murine HR23B protein, 9.5 mg/ml of enzyme domains in a 1:1 ratio, vapour diffusion method, against reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 28-32% PEG 4000, 0.2 M sodium acetate, heavy atom derivatization by soaking in 1 mM ethyl mercury thiosalicylate, 1 mM K2[PtCN4], or 1 mM KAuCN2 for 4 h, cryoprotection by 20% glycerol, X-ray diffraction structure determination and analysis at 1.85 A resolution |
3.5.1.52 | purified wild-type PUB domain, also as selenomethionine variants, by sitting drop vapour diffusion method at 17°C, with a reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% PEG 4000, using 20% glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 1.6 A resolution, mutant L66M/L75M/L87M PUB domain are crystallized in 50% PEG 400, 0.1 M CHES, pH 9.5, 0.2 M NaCl, molecular replacement, X-ray diffraction structure determination and analysis at 1.9 A resolution |
3.5.1.52 | the crystal structure of PNGase in complex with N,N'-diacetylchitobiose is described, refined at 3.4 A |