EC Number   |
|---|
   3.4.21.34 | apoenzyme and in complex with mupain-1-16, sitting drop vapor diffusion method, using 25% (w/v) poly(ethylene glycol) 3350, 0.1 M Tris-HCl pH8.5, 0.1 M NaCl |
| 3.4.21.34 | enzyme bound to inhibitor, hanging drop vapor diffusion method, using 0.2 M potassium dihydrogen phosphate, 23% (w/v) PEG3350 |
| 3.4.21.34 | in complex with human IgG1 monoclonal antibody DX-2930, hanging drop vapor diffusion method, using 0.1 M Bis-Tris, pH 6.5, 20% (w/v) PEG 5000 MME |
| 3.4.21.34 | protease domain recombinantly expressed in Pichia pastoris or in the baculovirus/Sf9 system. Structure for the endoglycosidase H-deglycosylated protease domain produced from Pichia pastoris is determined at 1.4 A. Structure for the mutagenically deglycosylated form produced from Sf9 cells is determined at 1.4 A |
| 3.4.21.34 | sitting drop vapor diffusion method, using 0.1 M carboxylic acids, 0.1 M HEPES/MOPS pH 7.5, 12.5% (v/v) 2-methyl-2,4-pentanediol, 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350 and a drop containing 0.1 M carboxylic acids, 0.1 M Tris/Bicine pH 8.5, 20% (w/v) PEG 500 MME |
