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EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.5crystallization in presence of a octapeptide inhibitor, sitting-drop vapor-diffusion procedure, 2.0 A resolution, indication of a ligand-dependent hinge movement mechanism 669857
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.5purified enzyme free or in complex with natural microbial tripeptide product K-26, hanging drop vapour diffusion method, mixing of 10 mg/ml protein in 50 mM Tris, pH 8.0, and 150 mM NaCl, with reservoir solution consisting of 0.2 M MgCl2, 0.1 M sodium cacodylate, pH 6.5, and 20% PEG 8000, and equilibration over reservoir solution, at 16°C for 2 days. For the complex with K-26, the protein is preincubated with 5 mM K-26 at room temperature for 1 h before crystallisation. X-ray diffraction structure determination and analysis at 1.80-1.75 A resolution 753554
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