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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.2.2.19in complex with product ADP-ribose. The interactions of the adenosine and proximal ribose occur primarily through quasidomain D due to a relative movement of the adenosine moiety by 8.4 A and a rotation of about 105°. Comparison with ADP-ribose glycohydrolase ARH3
Display the word mapDisplay the reaction diagram Show all sequences 3.2.2.19modeling of structure
Display the word mapDisplay the reaction diagram Show all sequences 3.2.2.19purified recombinant hARH1 in complex with K+ and ADP, mixing of equal volumes of protein solution containing 15-20 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl and 3 mM DTT with reservoir solution containing 150 mM lithium acetate and 25% w/v PEG 3350, at 20°C, equilibration over 0.75 ml reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution
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