3.2.2.19 | purified recombinant hARH1 in complex with K+ and ADP, mixing of equal volumes of protein solution containing 15-20 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl and 3 mM DTT with reservoir solution containing 150 mM lithium acetate and 25% w/v PEG 3350, at 20°C, equilibration over 0.75 ml reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution |