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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.76crystallized from different solution using the hanging-drop crystallization method
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.76purified recombinant enzyme, hanging drop vapour diffusion method, 4 mg/ml protein, mixing of 0.008 ml of both protein solution and reservoir solution, the latter containing 18% w/v PEG 3350, 0.18 M K/Na-tartrate, 3% w/v PEG MME 5000, 0.02 M ammonium sulfate, and 0.01 M MES-Na, pH 6.5, 25°C, 2-3 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, mercury-derivative crystals by single-wavelength anomalous dispersion data, Hg peak wavelength
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.76structure analysis of enzyme-substrate complex, PDB IDs 3W81 and 3W82
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