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Results 1 - 7 of 7
EC Number Crystallization (Commentary) Reference
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12hanging drop vapor diffusion method, using 33% (w/v) PEG 300, 0.1 M sodium citrate pH 5.0, 13% ethylene glycol and 10 mM CoCl2 730793
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12purified apo-active enzyme and trehalose-6-phosphate-complexed enzyme, vapor diffusion technique, mixing of 0.001 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 7.0, 50 mM NaCl, 1 mM MgCl2, and 10 mM DTT, with 0.001 ml of reservoir solution containing 20 mM sodium acetate trihydrate, pH 4.6, 50 mM CaCl2, 5% 2-propanol, 160 mM ammonium formate, and 16% PEG 3350, microseeding strategy, for substrate-bound crystals, soaking in 50 mM trehalose-6-phosphate for 6 to 12 h, 7 days, 20°C, X-ray diffraction structure detremination and analysis at 2.6 A and 1.7 A resolution, respectively 750507
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12purified detagged recombinant selenomethionine-labeled or wild-type enzyme, hanging drop vapour diffusion method, from 0.2 M MgCl2, 20% PEG 6000, 0.1 M 2-(N-morpholino)ethane sulfonic acid, pH 6.0, 1-2 weeks, X-ray diffraction structure determination and analysis at 1.9 A and 2.3 A resolution, respectively, and heavy atom derivatization using soaking procedures, molecular replacement 750510
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12purified isolated N-terminal domain of Tps2 (Tps2NTD, residues 1-534), X-ray diffraction structure determination and analysis at 2.56 A resolution, molecular replacement using the structure of Escherichia coli OtsA, a Tps1 homologue, PDB ID code 1UQU, as a search model. Purified recombinant Tps2 C-terminal trehalose-6-phosphate phosphatase domain (Tps2PD) bound to BeF3 and trehalose (Tps2PD-BeF3-trehalose transition-state complex), X-ray diffraction structure determination and analysis at 2.0 A resolution 752047
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12purified recombinant catalytically dead Tps2PD(D24N) from Cryptococcus neoformans bound to trehalose-6-phosphate, X-ray diffraction structure determination and analysis at 2.56 A resolution 752047
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12sitting drop vapor diffusion crystallization of both the native and SeMet proteins 749237
Show all pathways known for 3.1.3.12Display the reaction diagram Show all sequences 3.1.3.12sitting drop vapour diffusion method with 30% PEG 4000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5) 682725
Results 1 - 7 of 7