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EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.21.B2crystallization experiments with the mutant enzyme R32A/E63A-nonspecific dsDNA complex are performed using the sitting-drop vapour-diffusion method at 20°C. The crystals of the mutant enzyme R32A/E63A-nonspecific dsDNA complex are obtained using a reservoir solution of 0.2 M calcium acetate, 0.1 M imidazole (pH 8.0), and 10% PEG 8000.The structure of enzyme in complex with double-stranded DNA without the R.PabI recognition sequence is determined by X-ray crystallography. The 1.9 A resolution structure of the complex shows that the enzyme forms a tetrameric structure to sandwich the double-stranded DNA and the tetrameric structure is stabilized by four salt bridges 746460
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