EC Number |
---|
3.1.1.43 | - |
3.1.1.43 | 0.0015 ml of concentrated AEH is mixed with an equal volume of 12-15% polyethylene glycol 8000 in 100 mM cacodylate, pH 6.5, and equilibrated against 0.5 ml of this precipitant solution, crystals diffract to 1.9A |
3.1.1.43 | 5 mg/ml AEH in 50 mM sodium phosphate, pH 6.2 is mixed with 0.003 ml 15-17% polyethylene glycol 4000, 200 mM ammonium acetate, 100 mM sodium acetate, ph 4.5, followed by equilibration agains this polyethylene glycol solution, crystals diffract to 2.0 A |
3.1.1.43 | the crystal structures of the native protein, the S122A and D123N mutants, and the native enzyme in complex with L-tyrosine amide are solved at resolutions of 1.75 A, 1.90 A, 1.85 A and 1.90 A, respectively |