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Results 1 - 6 of 6
EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.41- 652917
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.41crystal structure of the P228A variant is determined at 2.1 A resolution. The replacement does not affect the overall secondary, tertiary, and quaternary structures and moderately decreases the thermal stability 749261
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.41hanging drop method, crystal structure of the P228A variant of Thermotoga maritima acetyl esterase, crystal structure of the Thermotoga maritima acetyl esterase complex with a substrate analog 746975
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.41in complex with 2-(2-oxo-1,3-dihydroindol-3-yl) acetate, hanging drop vapor diffusion method, using 0.05 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5 and 30% (v/v) pentaerythritolethoxylate 746975
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.41mutant enzyme P228A, hanging drop vapor diffusion method, using 0.05M ammonium sulfate, 0.1 M Bis-Tris pH 6.5 and 30% (v/v) pentaerythritolethoxylate 749261
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.41purified recombinant selenomethionine-substituted and wild-type enzyme in complex with covalently bound phenylmethylsulfonyl fluoride and paraoxon, hanging drop vapour diffusion method, for selenomethionine-substituted enzyme: mixing of 500 nl of 15 mg/ml protein in 20 mM Tris pH 7.9, 150 mM NaCl, 0.25 mM TCEP, in presence or absence of inhibitors, with 500 nl of reservoir solution containing 20% w/v PEG-3000, 0.1 M HEPES pH 7.5, 0.2 M NaCl, and equilibration against 0.25 ml of reservoir solution, for wild-type enzyme: mixing of 100 nl of 22.8 mg/ml protein in 20 mM Tris pH 7.9, 150 mM NaCl, 0.25 mM TCEP, in presence or absence of inhibitors, with 100 nl of reservoir solution containing 0.2 M calcium acetate hydrate, 20% w/v PEG 3350, pH 7.3, and equilibration against 0.06 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.4 A and 2.1 A resolution, respectively, molecular replacement, structure modeling 730907
Results 1 - 6 of 6