EC Number |
Reference |
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2.7.7.B22 | cocrystal structures of the Hermes transposase bound to DNA that mimics the reaction step immediately prior to hairpin formation. A large DNA conformational change occurs between the initial cleavage step and subsequent hairpin formation that changes which strand is acted upon by a single active site. The complement of divalent metal ions bound by the catalytically essential DDE residues, and the identity of the -2 flanking base pair affect the conformationbal change. For efficient hairpin formation an A:T basepair is favored at the -2 position. The histidine residue within a conserved C/DxxH motif interacts directly with the scissile phosphate |
762045 |
2.7.7.B22 | cryo-EM structures of piggyBac transpososomes show a synaptic complex with hairpin DNA intermediates and a strand transfer complex capturing the integration step. The excised TTAA hairpin intermediate and the TTAA target adopt essentially identical conformations. The transposase forms an asymmetric dimer in which the two central domains synapse the ends while two C-terminal domains form a separate dimer that contacts only one transposon end. In the strand transfer structure, target DNA is severely bent and the TTAA target is unpaired |
761979 |
2.7.7.B22 | hanging drop vapor diffusion method at 24°C, crystal structure of the enzyme in both Mn2+-bound and Mn2+-free forms |
725373 |
2.7.7.B22 | hanging-drop vapour-diffusion method, crystallized at 24°C using a reservoir solution consisting of 100 mM Na HEPES pH 7.5 and 20%(v/v) ethanol. X-ray diffraction data are collected to 1.78 A. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 65.00, b = 34.07, c = 121.58 A, alpha = 90, beta = 100.20, gamma = 90 |
723834 |
2.7.7.B22 | puriifed recombinant tagged transposase catalytic domain SB100X, residues 114-340, by hanging drop vapour diffusion method, mixing of 0.002 ml of 19 mg/ml protein in 20 mM HEPES, pH 7.2, 50 mM NaCl, 2% v/v glycerol, 10 mM MgCl2, and 0.2 mM TCEP, with 0.002 ml of precipitant solution containing 0.1 M Na-citrate, pH 4.8, and 3.2 M ammonium sulfate, 4°C, 5 days, X-ray diffraction structure determination and analysis at 1.40 A resolution, PDB ID 3HOS |
739149 |
2.7.7.B22 | the C-terminal cysteine-rich domain, PB(552-594), forms a monomeric folded Zn2+-complexed structure |
762046 |