2.7.7.47 | wild type enzyme complexed with ATP, magnesium, and streptomycin, sitting drop vapor diffusion method, using 10% (w/v) PEG 20,000, 20% (v/v) PEG monomethyl ether 550, 0.1 M MOPS/HEPES-Na, pH 7.5. Mutant enzyme E87Q complexed with ATP and streptomycin or dihydrostreptomycin, sitting drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.2 M CaCl2, and 0.1 M HEPES, pH 7.5 |