EC Number   |
|---|
   2.7.11.26 | by hanging-drop vapour-diffusion method, crystals belong to space group P212121, with unit-cell parameters a = 55.6, b = 113.7, c = 117.3 A, alpha = beta = gamma = 90.0°, to 2.9 A resolution |
| 2.7.11.26 | enzyme complexed with the Cdk2 inhibitor WHI-P180, using 0.1 M Tris pH 8.5, 10% (v/v) glycerol, 2.0 M sodium/potassium phosphate at 4°C |
| 2.7.11.26 | hanging drop vapour-diffusion method, prismatic crystals, orthorhombic space group P2(1)2(1)2(1), unit cell parameters a = 82.9, b = 86.1, c = 178.1 A |
| 2.7.11.26 | isoform TTBK2 kinase domain with ADP, using 0.1 M Tris pH 8.5, 10% (v/v) glycerol and 2 M Na/K phosphate |
| 2.7.11.26 | purified recombinant Leu-Glu-His6-tagged GSK3beta in complex with ATP or ATP analogue AMP-PNP, 10 mg/ml protein, 2 mM ATP or AMP-PNP, 12-14% w/v PEG 6000, 100 mM NaCl, 5 mM MgCl2, 10% v/v glycerol, in 100 mM HEPES-NaOH, pH 7.5, hanging drop vapor diffusion method, 4°C, several days, soaking in 0.1 mM ethylmercuric thiosalicylate at pH 7.5 for 1 h, cryoprotection by 30% w/v D-sorbitol, X-ray diffraction structure determination and analysis at 1.7-2.6 A resolution |
