EC Number   |
|---|
    2.7.1.48 | analysis of cytidine-complex structure of hsUCK2 (PDB ID 1UEJ) |
| 2.7.1.48 | crystals of uridine kinase 2 and in complex with CTP, UTP, cytidine, ATPgammaS and with both cytidine and ATP, the latter complex diffracted to 1.8 A resolution |
| 2.7.1.48 | in complex with cytidine and beta,gamma-methyleneadenosine 5'-triphosphate, sitting drop vapor diffusion method, using 0.1 M NaCl, 0.1 M bicine, pH 9.0, and 20% (w/v) polyethylene glycol monomethyl ether 550 |
| 2.7.1.48 | in complex with Mg2+, ADP, and CMP by sitting drop vapor diffusion method |
| 2.7.1.48 | purified recombinant enzyme H93Y variant ttCK free or incomplex with CMP, sitting-drop vapor diffusion method, mixing of 500 nl of 25 mg/ml protein, with 1 mM CMP, 1 mM ADP, and 1 mM MgCl2 in case of the enzyme complex, with 500 nl well solution containing 67% 40% v/v isopropanol, 15% w/v PEG 8000, and 0.1 M imidazole, pH 6.5, and 33% distilled water for the free enzyme, and containing 50% w/v PEG 200, and 0.1 M Tris-HCl, pH 8.0, for the enzyme complex, 20°C, X-ray diffraction structure determination and analysis at 2.25 A resolution, molecular replacement |
| 2.7.1.48 | purified recombinant Y93H ttCK in complex with cytidine and AMPPCP, X-ray diffraction structure determination and analysis at 2.49 A resolution |
| 2.7.1.48 | truncated form of UCK2 (1-250) in complex with substrates or feedback inhibitors UTP and CTP |
