2.6.1.B6 | hanging drop method of vapor diffusion. The structure of DesI is solved in complex with its product, dTDP-4-amino-4,6-dideoxyglucose, to a nominal resolution of 2.1 A. Each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands. Crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions of 59.5 A, 66.8 A, 242.0 A and one dimer per asymmetric unit. Crystal dimensions are typically 1 mm * 0.6 mm * 0.2 mm |
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