  2.6.1.B18 | purified recombinant wild-type or SeMet-labeled enzyme in complex with preQ0, hanging drop vapour diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution containing 17-19% PEG 3350, 0.15 M potassium thiocyanate, and 0.05% sodium azide, and equilibration against 0.5 mL of reservoir solution, for the SeMet-labeled protein in complex with preQ0, the same conditions are used, except crystallization is conducted in sitting drops set up by mixing 0.002 ml of protein solution with 0.001 ml of reservoir solution, 20°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 1.91 A and 2.74 A resolution, respectively, modelling |
