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Results 1 - 10 of 10
EC Number Crystallization (Commentary) Reference
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9crystallization of enzyme complexed with 6-carboxyethyl-7-oxo-8-ribityllumazine, sitting drop vapour diffusion method against equal amounts of reservoir solution containing 0.1 M bicine, pH 9.0, 65% v/v 2-mehyl-2,4-pentanediol, enzyme solution: 9 mg/ml, 20 mM TrisHCl, pH 7.0, 0.1 M KCl, 10 molar excess of solid 8, X-ray structure determination and analysis, structure model building and refinement, overview 637584
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9crystals of wild-type enzyme are grown at 18°C using the sitting drop vapor diffusion method by mixing equal amounts of protein (6 mg/ml) in 100 mM potassium phosphate, pH 7.0, containing 30 mM Tris and 2 mM dithiothreitol with a reservoir solution containing 0.1 M HEPES, pH 7.0, and 40% 2-methyl-2,4-pentanediol. Crystal structures of the enzyme and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine 727855
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9detailed structure determination and analysis of enzyme complexed with heavy atoms, three-dimensional structure model 637568
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9from 1.3 M sodium/potassium phosphate, pH 8.7, 0.35 mM 5-nitroso-6-(1'-D-ribitylamino)-2,4(1H,3H)-pyrimidinedione, X-ray structure determination and analysis 637566
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9hanging drop vapor diffusion method, using 12% (w/v) PEG 8000, 10% (w/v) glycerol, 0.5 M potassium chloride 737344
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9large crystals by vapour diffusion method, initial solution: 0.7 M sodium/potassium phosphate, pH 8.7, 0.3 mM 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, protein 2 mg/ml, reservoir solution: 1.3 M sodium/potassium phosphate, pH 8.7, X-ray structure determination and analysis 637571
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9modeling for the binding of two molecules of inhibitor 5-(4-phosphonobutyryl)amino-6-D-ribitylaminouracil in the active site 637586
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9molecular modeling of inhibitors to the active site 637585
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9purified recombinant homodimeric N-terminal enzyme domain in complex with riboflavin, 7 mg/ml protein in 70 mM sodium potassium phosphate, pH 7.0, and 100 mM sodium chloride, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular modeling 659699
Show all pathways known for 2.5.1.9Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.9structure determination by multiwavelength anomalous diffraction method, modeling 637582
Results 1 - 10 of 10