EC Number   |
Reference |
|---|
    2.5.1.15 | - |
663405, 759244 |
| 2.5.1.15 | a complex of the purified bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase with a pterin monophosphate substrate analogue, structure solved by molecular replacement and refined to 2.3 A resolution |
662648 |
| 2.5.1.15 | an inhibitor is soaked into the apo crystals of the enzyme and the structure of the complex is determined to 2.1 A resolution. Thed inhibitor occupies both the pterin and 4-aminobenzoate binding pocket of the enzyme |
758913 |
| 2.5.1.15 | apo-enzyme, and enzyme in complex with HPPK substrate 6-hydroxymethyl-7,8-dihydropterin or inhibitor 2-(7-amino-1-methyl-4,5-dioxo-1,4,5,6-tetrahydorpyrimido[4,5-c]pyridazin-3-yl)propanoic acid, X-ray diffraction structure determination and analysis at 2.2-2.3 A resolution |
723522 |
| 2.5.1.15 | apo-structure and structure of the complex with pteroate, analysis, overview |
723771 |
| 2.5.1.15 | apoenzyme (PDB: 2VEF), complex with 6-hydroxymethyl-7,8-dihydropterin monophosphate (DHPP) (PDB: 2VEG), TIM alpha/beta barrel fold with highly conserved 6-hydroxymethyl-7,8-dihydropterin diphosphate-binding pocket, crystals: space group P2(1)2(1)2(1), unit cell parameters: a: 45, b: 90, c: 137, loop 1 and 2 highly flexible, dimer of two identical monomers in the asymmetric unit, in complex with DHPP only one monomer of the dimer has substrate bound wide-scale rearrangement of active site upon 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPPP) binding mediated by diphosphate moiety, hanging-drop method: 2 microlitre protein solution (13 mg/ml) + 2 microl precipitant (0.2 M ammonium iodide, 20% (w/v) poly(ethylene glycol) 3350) +/-2.5 mM DHPPP (hydrolysis to DHPP during crystallization), 7-14 days, molecular replacement-based structure determination |
690820 |
| 2.5.1.15 | hanging-drop vapor-diffusion method at 20°C, crystal structure of the enzyme in complex with four substrates/analogs |
759490 |
| 2.5.1.15 | HPPK-DHPS from wild type and sulfa-resistant parasites, in complex with pteroate, sulfathiazole-dihydropteroate, and sulfadoxine-dihydropteroate, microbatch method, using 0.1 mM bicine pH 9.0, 0.5-0.6 M calcium acetate-Ca(OAc)2, 20% (w/v) PEG 4000 at 25°C |
759184 |
| 2.5.1.15 | in complex with 6-hydroxymethylpterin diphosphate, pterin, the ATP analog AMPCPP, and 4-aminobenzoate, at 20°C, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M potassium citrate tribasic monohydrate |
759490 |
| 2.5.1.15 | in complex with inhibitors |
759069 |
