2.4.99.19 | purified detagged and methylated wild-type and selenomethionine-labeled C-terminal globular domain of PglB, hanging drop vapour diffusion method, 500 nl of 10 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed in an 1:1 ratio with reservoir solution containing 0.1 M sodium cacodylate, pH 6.5, 18% PEG 8000, 0.2 M calcium acetate, 20°C, X-ray diffraction structure determination and analysis at 2.8 A resolution |
709145 |