2.3.1.212 | purified enzyme, sitting drop vapour diffusion method, mixing of 500 nl of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, and 2 mM DTT, with 500 nl of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 15% w/v PEG 8000, and 200 mM potassium thiocyanate, and equilibration against 0.1 ml of reservoir solution, 3 days, cryoprotection by 20% v/v glycerol in reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution |
721184 |