   2.3.1.211 | purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, optimization of the crystallization conditions, mixing 0.0005 ml of protein solution, containing 10 mg/ml in 20 mM HEPES-NaOH, pH 7.0, 100 mM NaCl, and 2 mM DTT, with an equal volume of reservoir solution, containing 100 mM HEPES-NaOH, pH 7.8, 1995 mM ammonium sulfate, 3% dimethylsulfoxide, and 2 mM CoA, and equilibration against 0.1 ml reservoir solution, 20°C, cryoprotection in 100 mM HEPES-NaOH pH 7.8, 1330 mM ammonium sulfate, 2 mM CoA, 12% v/v glycerol and 14% w/v PEG 400, X-ray diffraction structure determination and analysis st 2.5 A resolution |
721196 |
