EC Number   |
Reference |
|---|
    2.1.2.2 | - |
485729, 485731, 485738, 485741 |
| 2.1.2.2 | crystals grown at 20°C by hanging-drop vapor diffusion, space group C222, a = 140.9 A, b = 97.6 A, c = 102.4 |
485719 |
| 2.1.2.2 | crystals grown by vapor diffusion, space group C222, a = 140.5 A, b = 98.2 A, c = 103.3 A |
485715 |
| 2.1.2.2 | diffraction to 2.5 A, space group P3121 |
701528 |
| 2.1.2.2 | in complex with magnesium and iodide at 2.2 A resolution, and with cofactor analogue 5-methyltetrahydrofolate at 2.2 A resolution |
705147 |
| 2.1.2.2 | orthorhombic space group C222, a = 141.4 A, b = 98.2 A, c = 103.5 A |
485720 |
| 2.1.2.2 | purified recombinant enzyme in apoform or in complex with inhibitors 10S methylthio-DDACTHF and 10R methylthio-DDACTHF, mixing of 0.002 ml of 15 mg/mL in 20 mM Tris, pH 8.0, 200 mM NaCl, 5 mM DTT, and inhibitors in a 5fold molar excess for complex crystals, with 0.002 ml of reservoir solution containing 0.1 M phosphate/citrate buffer, 1.5-2.0 M ammonium sulfate at pH 4.2, X-ray diffraction structure determination and analysis at 1.52-1.70 A resolution, molecular replacement |
735672 |
| 2.1.2.2 | purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 22 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M lithium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 14% PEG 3350, and equilibration against 0.2 ml of reservoir solution, 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement |
718507 |
| 2.1.2.2 | sitting drop vapor diffusion method, using 0.1 M Tris-HCl (pH 7.5), 0.333 M NaCl, 18% (w/v) polyethylene glycol (PEG) 4000, and a 2% (w/v) PEG 400 |
756039 |
| 2.1.2.2 | vapor diffusion sitting drop method, crystals of enzyme in complex with 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid |
658000 |
