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Results 1 - 7 of 7
EC Number Crystallization (Commentary)
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.12-
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.123.3 A crystal structure of the entire PSI super-complex, sitting drop variant of the vapor-diffusion technique at 4°C
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.12highly ordered two-dimensional crystals of photosystem I reaction center complex
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.12isolation and reconstitution of the functional PSI complexes into 2D arrays. The solubilized and crystallized PSI has essentially the same protein composition, as shown by SDS/polyacrylamide gels
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.12purified plant photosystem I supercomplex including light-harvesting complex I, sitting drop vapour diffusion technique, mixing of 0.006-0.008 ml of PSI protein solution with an equal volume of reservoir solution containing 50 mM di-potassium phosphate, 50 mM Tris, pH 8, 12-17% PEG 400, 1% glycerol, 2 mM L-glutathione, and 0.03% octyl glucose neopentyl glycol, and equilibration against 0.5 ml of reservoir solution, 4°C, 1 month, X-ray diffraction structure determination and analysis at 2.6 A resolution, single-wavelength anomalous diffraction phasing of PSI-LHCI
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.12single crystals from a complex of photosystem I with ferredoxin are grown using PEG 400 and CaCl2 as precipitation agents. The crystals diffract X-rays to a resolution of 7–8 A. The space group iss determined to be orthorhombic with the unit cell dimensions a = 194 A, b = 208 A, and c = 354 A. The crystals contain photosystem I and ferredoxin in a 1:1 ratio. Electron paramagnetic resonance (EPR) measurements on these crystals are reported, where EPR signals of the three [4Fe-4S] clusters FA, FB, FX, and the [2Fe-2S] cluster of ferredoxin are detected
Show all pathways known for 1.97.1.12Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.12sitting-drop variant of the vapour-diffusion technique at 4°C, crystals diffract to 4 A resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P21, with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 A, beta = 90.484
Results 1 - 7 of 7